Smithsonian Institution Archives

The History of the Cell Sorter Videohistory Collection, 1991

1 results in SIA.FARU9554 for "Genetic engineering"
Recorded at Stanford University, Palo Alto, California, featured the Herzenbergs, Krasnow, Montano, Moore, Nozaki, Parks, Stovel, Sweet, and Veizades discussing the development of the FACS machine at Stanford, history of its improvements, and current research uses; c. 1955-1991, including: Description of the activities at the Shared FACS Facility; explanation of the laser system and electronics of the FACS II; description of the functions of the FACStar Plus and FACScan machines; current research applications of cell sorting technology in the Stanford Department of Genetics, particularly in AIDS research; Drosophila whole animal cell sorting project; the Herzenbergs' biographical information, including experiences at the Pasteur Institute; discovery of monoclonal antibodies and their application for cell sorting; experiments using glutathione as a treatment for AIDS patients; atmosphere in the Herzenberg laboratory; history of the engineering design and construction of FACS I; adaptation of ink jet printing technology for cell sorting; current engineering modifications to FACS technology; history of the collaboration between BD and Stanford; challenges of manufacturing early commercial sorters; success of FACS I and demand for the machines by the biomedical community; development of electronics for FACS machines; early computing capabilities; and development of computer programs for subsequent generations of FACS machines. Visual documentation included: Visual tour of the offices of the Shared FACS Facility; close-ups of the FACS II machine, particularly the laser system; close-up of the FACStar Plus and FACScan machines; computer graphics generated from cell analysis on a monitor; close-up of the advanced pattern sorting FACS prototype; views of students working in the Herzenberg laboratory; photographs of Drosophila embryo cells; photograph of cell stream forming into droplets; molds for making valves and connector for the early FACS machine; FACS I valve box; and photos of associates of the Herzenberg laboratory.